GPX4 antibody (AA 30-197)

Catalog No. ABIN7601212

The Mouse Monoclonal anti-GPX4 antibody has been validated for WB and FACS. It is suitable to detect GPX4 in samples from Human.

Quick Overview for GPX4 antibody (AA 30-197) (ABIN7601212)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 6I4E7

Product Details anti-GPX4 Antibody

Binding Specificity: AA 30-197

Purpose: Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7) (ABIN7601212). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Esworthy RS, Doan K, Doroshow JH, Chu FF (July 1994). "Cloning and sequencing of the cDNA encoding a human testis phospholipid hydroperoxide glutathione peroxidase". Gene 144 (2): 317-8. 2. Smith AC, Mears AJ, Bunker R, Ahmed A, MacKenzie M, Schwartzentruber JA, Beaulieu CL, Ferretti E, Majewski J, Bulman DE, Celik FC, Boycott KM, Graham GE (2014). "Mutations in the enzyme glutathione peroxidase 4 cause Sedaghatian-type spondylometaphyseal dysplasia". Journal of Medical Genetics 51 (7): 470-4.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: GPX4

Background:

Synonyms: Desmin, DES

Background: Glutathione peroxidase 4, also known as GPX4, is an enzyme that in humans is encoded by the GPX4 gene. This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants.

Molecular Weight: 19 kDa

Gene ID: 2879

UniProt: P36969