NOP58 antibody (AA 30-450)

Catalog No. ABIN7601224

The Rabbit Polyclonal anti-NOP58 antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect NOP58 in samples from Human.

Quick Overview for NOP58 antibody (AA 30-450) (ABIN7601224)

Target: NOP58 (NOP58 Ribonucleoprotein (NOP58))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOP58 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-NOP58 Antibody

Binding Specificity: AA 30-450

Purpose: Anti-NOP58 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NOP58 Antibody Picoband® (ABIN7601224). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NOP58 recombinant protein (Position: K30-K450).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/8/2016. 2. Lyman, S. K., Gerace, L., Baserga, S. J. Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins. RNA 5: 1597-1604, 1999. 3. Nelson, S. A., Santora, K. E., LaRochelle, W. J. Isolation and characterization of a novel PDGF-induced human gene. Gene 253: 87-93, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NOP58

Target: NOP58 (NOP58 Ribonucleoprotein (NOP58))

Alternative Name: NOP58

Background:

Synonyms: Matrix metalloproteinase-16, MMP-16, 3.4.24.-, MMP-X2, Membrane-type matrix metalloproteinase 3, MT-MMP 3, MTMMP3, Membrane-type-3 matrix metalloproteinase, MT3-MMP, MT3MMP, MMP16, MMPX2,

Tissue Specificity: Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Background: Nucleolar protein 58 is a protein that in humans is encoded by the NOP58 gene. The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.

Molecular Weight: 70 kDa

Gene ID: 51602

UniProt: Q9Y2X3

Pathways: Protein targeting to Nucleus