HTRA2 antibody (AA 30-458)

Catalog No. ABIN7601225

This anti-HTRA2 antibody is a Rabbit Polyclonal antibody detecting HTRA2 in WB, ELISA, IHC, IF, FACS and ICC. Suitable for Human.

Quick Overview for HTRA2 antibody (AA 30-458) (ABIN7601225)

Target: HTRA2 (HtrA Serine Peptidase 2 (HTRA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTRA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-HTRA2 Antibody

Binding Specificity: AA 30-458

Purpose: Anti-HTRA2 Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-HTRA2 Antibody Picoband® (ABIN7601225). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HTRA2 recombinant protein (Position: R30-E458). Human HTRA2 shares 85.7% amino acid (aa) sequence identity with mouse HTRA2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bogaerts, V., Nuytemans, K., Reumers, J., Pals, P., Engelborghs, S., Pickut, B., Corsmit, E., Peeters, K., Schymkowitz, J., De Deyn, P. P., Cras, P., Rousseau, F., Theuns, J., Van Broeckhoven, C. Genetic variability in the mitochondrial serine protease HTRA2 contributes to risk for Parkinson disease. Hum. Mutat. 29: 832-840, 2008. 2. Chao, J.-R., Parganas, E., Boyd, K., Hong, C. Y., Opferman, J. T., Ihle, J. N. Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature 452: 98-102, 2008. Note: Erratum: Nature 452: 900 only, 2008. 3. Faccio, L., Fusco, C., Chen, A., Martinotti, S., Bonventre, J. V., Zervos, A. S. Characterization of a novel human serine protease that has extensive homology to bacterial heat shock endoprotease HtrA and is regulated by kidney ischemia. J. Biol. Chem. 275: 2581-2588, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HTRA2

Target: HTRA2 (HtrA Serine Peptidase 2 (HTRA2))

Alternative Name: HTRA2

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: Serine protease HTRA2, mitochondrial is an enzyme that in humans is encoded by the HTRA2 gene. This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Molecular Weight: 36 kDa

Gene ID: 27429

UniProt: O43464

Pathways: Positive Regulation of Endopeptidase Activity