SHISA7 antibody (AA 308-535)

Catalog No. ABIN7601243

Product Details anti-SHISA7 Antibody

Target: SHISA7 (Shisa Homolog 7 (SHISA7))

Binding Specificity: AA 308-535

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHISA7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Anti-SHISA7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SHISA7 Antibody Picoband® (ABIN7601243). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SHISA7 recombinant protein (Position: Y308-E535).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Han, W., Li, J., Pelkey, K. A., Pandey, S., Chen, X., Wang, Y.-X., Wu, K., Ge, L., Li, T., Castellano, D., Liu, C., Wu, L.-G., Petralia, R. S., Lynch, J. W., McBain, C. J., Lu, W. Shisa7 is a GABAA receptor auxiliary subunit controlling benzodiazepine actions. Science 366: 246-250, 2019. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 1/30/2017. 3. Pei, J., Grishin, N. V. Unexpected diversity in Shisa-like proteins suggests the importance of their roles as transmembrane adaptors. Cell. Signal. 24: 758-769, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SHISA7

Target: SHISA7 (Shisa Homolog 7 (SHISA7))

Alternative Name: SHISA7 (SHISA7 Products)

Background:

Synonyms: Placenta-specific protein 9, PLAC9

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: Shisa7, a single-passing transmembrane protein, localizes at GABAergic inhibitory synapses and interacts with GABA-A receptors. Shisa7 controlled receptor abundance at synapses and sped up the channel deactivation kinetics. Shisa7 also potently enhanced the action of diazepam, a classic benzodiazepine, on GABA-A receptors. Genetic deletion of Shisa7 selectively impaired GABAergic transmission and diminished the effects of diazepam in mic

Molecular Weight: 70 kDa

Gene ID: 729956