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Acetyl-CoA Carboxylase alpha antibody (AA 31-2336)

The Rabbit Polyclonal anti-Acetyl-CoA Carboxylase alpha antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect Acetyl-CoA Carboxylase alpha in samples from Human.
Catalog No. ABIN7601254

Quick Overview for Acetyl-CoA Carboxylase alpha antibody (AA 31-2336) (ABIN7601254)

Target

See all Acetyl-CoA Carboxylase alpha (ACACA) Antibodies
Acetyl-CoA Carboxylase alpha (ACACA)

Reactivity

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  • 46
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  • 6
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  • 1
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Human

Host

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Rabbit

Clonality

  • 90
  • 14
Polyclonal

Conjugate

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  • 1
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  • 1
This Acetyl-CoA Carboxylase alpha antibody is un-conjugated

Application

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  • 9
  • 3
  • 1
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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Binding Specificity

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    AA 31-2336

    Purpose

    Anti-ACACA Antibody Picoband®

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins.

    Characteristics

    Anti-ACACA Antibody Picoband® (ABIN7601254). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human ACACA recombinant protein (Position: D31-R2336).

    Isotype

    IgG
  • Application Notes

    Western blot, 0.25-0.5 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Abu-Elheiga, L., Brinkley, W. R., Zhong, L., Chirala, S. S., Woldegiorgis, G., Wakil, S. J. The subcellular localization of acetyl-CoA carboxylase 2. Proc. Nat. Acad. Sci. 97: 1444-1449, 2000. 2. Abu-Elheiga, L., Jayakumar, A., Baldini, A., Chirala, S. S., Wakil, S. J. Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms. Proc. Nat. Acad. Sci. 92: 4011-4015, 1995. 3. Abu-Elheiga, L., Matzuk, M. M., Kordari, P., Oh, W., Shaikenov, T., Gu, Z., Wakil, S. J. Mutant mice lacking acetyl-CoA carboxylase 1 are embryonically lethal. Proc. Nat. Acad. Sci. 102: 12011-12016, 2005.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Storage

    4 °C,-20 °C

    Storage Comment

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • Target

    Acetyl-CoA Carboxylase alpha (ACACA)

    Alternative Name

    ACACA

    Background

    Synonyms: Serine protease HTRA1, High-temperature requirement A serine peptidase 1, L56, Serine protease 11, HTRA1, HTRA, PRSS11

    Tissue Specificity: Widely expressed, with strongest expression in placenta (at protein level). Secreted by synovial fibroblasts. Up- regulated in osteoarthritis and rheumatoid arthritis synovial fluids and cartilage as compared with non-arthritic (at protein level).

    Background: Acetyl-CoA carboxylase 1 also known as ACC-alpha or ACCa is an enzyme that in humans is encoded by the ACACA gene. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

    Molecular Weight

    266 kDa

    Gene ID

    31

    UniProt

    Q13085

    Pathways

    AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effect
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