RSPH3 antibody (AA 31-473)

Catalog No. ABIN7601273

Product Details anti-RSPH3 Antibody

Target: RSPH3 (Radial Spoke 3 Homolog (Chlamydomonas) (RSPH3))

Binding Specificity: AA 31-473

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RSPH3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purpose: Anti-RSPH3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RSPH3 Antibody Picoband® (ABIN7601273). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RSPH3 recombinant protein (Position: Q31-H473).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Jeanson, L., Copin, B., Papon, J.-F., Dastot-Le Moal, F., Duquesnoy, P., Montantin, G., Cadranel, J., Corvol, H., Coste, A., Desir, J., Souayah, A., Kott, E., and 9 others. RSPH3 mutations cause primary ciliary dyskinesia with central-complex defects and a near absence of radial spokes. Am. J. Hum. Genet. 97: 153-162, 2015. 2. Jivan, A., Earnest, S., Juang, Y.-C., Cobb, M. H. Radial spoke protein 3 is a mammalian protein kinase A-anchoring protein that binds ERK1/2. J. Biol. Chem. 284: 29437-29445, 2009. 3. Koukoulas, I., Augustine, C., Silkenbeumer, N., Gunnersen, J. M., Scott, H. S., Tan, S.-S. Genomic organisation and nervous system expression of radial spoke protein 3. Gene 336: 15-23, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RSPH3

Target: RSPH3 (Radial Spoke 3 Homolog (Chlamydomonas) (RSPH3))

Alternative Name: RSPH3 (RSPH3 Products)

Background:

Synonyms: Protein eva-1 homolog A, Protein FAM176A, Transmembrane protein 166, EVA1A, FAM176A, TMEM166, SP24

Tissue Specificity: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.

Background: Radial spoke head protein 3 homolog (RSPH3), also known as radial spoke head-like protein 2 (RSHL2), is a protein that in humans is encoded by the RSPH3 gene. The protein encoded by this gene acts as a protein kinase A anchoring protein. Mutations in this gene cause primary ciliary dyskinesia, a disorder characterized by defects of the axoneme in motile cilia and sperm flagella. The homolog of this gene was first identified in the blue-green algae Chlamydomonas as encoding a radial spoke protein that formed a structural component of motile cilia and flagella. Alternate splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 70 kDa

Gene ID: 83861