STK3 antibody (AA 314-456)

Catalog No. ABIN7601288

This anti-STK3 antibody is a Rabbit Polyclonal antibody detecting STK3 in WB, IHC, ELISA, IF, ICC and FACS. Suitable for Human and Monkey.

Quick Overview for STK3 antibody (AA 314-456) (ABIN7601288)

Target: STK3 (serine/threonine Kinase 3 (STK3))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-STK3 Antibody

Binding Specificity: AA 314-456

Purpose: Anti-STK3/MST-2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-STK3/MST-2 Antibody Picoband® (ABIN7601288). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human STK3/MST-2 recombinant protein (Position: E314-D456).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Creasy, C. L., Chernoff, J. Cloning and characterization of a member of the MST subfamily of Ste20-like kinases. Gene 167: 303-306, 1995. 2. Du, X., Wen, J., Wang, Y., Karmaus, P. W. F., Khatamian, A., Tan, H., Li, Y., Guy, C., Nguyen, T.-L. M., Dhungana, Y., Neale, G., Peng, J., Yu, J., Chi, H. Hippo/Mst signalling couples metabolic state and immune function of CD8-alpha+ dendritic cells. Nature 558: 141-145, 2018. 3. Harvey, K. F., Pfleger, C. M., Hariharan, I. K. The Drosophila Mst ortholog, hippo, restricts growth and cell proliferation and promotes apoptosis. Cell 114: 457-467, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for STK3

Target: STK3 (serine/threonine Kinase 3 (STK3))

Alternative Name: STK3

Background:

Synonyms: Cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta subunit, CD131, Csf2rb, Aic2b, Csf2rb1, Il3rb1

Tissue Specificity: Broadly expressed with higher expression in heart.

Background: Serine/threonine-protein kinase 3 is an enzyme that in humans is encoded by the STK3 gene. This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 56 kDa

Gene ID: 6788

UniProt: Q13188

Pathways: Tube Formation