Integrin beta 5 antibody (AA 32-689)

Catalog No. ABIN7601319

This Mouse Monoclonal antibody specifically detects Integrin beta 5 in WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for Integrin beta 5 antibody (AA 32-689) (ABIN7601319)

Target: Integrin beta 5 (ITGB5)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 9E2

Product Details anti-Integrin beta 5 Antibody

Binding Specificity: AA 32-689

Purpose: Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2) (ABIN7601319). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Integrin beta 5/ITGB5 recombinant protein (Position: S32-D689).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Busk, M., Pytela, R., Sheppard, D. Characterization of the integrin alpha-v-beta-6 as a fibronectin-binding protein. J. Biol. Chem. 267: 5790-5796, 1992. 2. McLean, J. W., Vestal, D. J., Cheresh, D. A., Bodary, S. C. cDNA sequence of the human integrin beta 5 subunit. J. Biol. Chem. 265: 17126-17131, 1990. 3. Reynolds, L. E., Wyder, L., Lively, J. C., Taverna, D., Robinson, S. D., Huang, X., Sheppard, D., Hynes, R. O., Hodivala-Dilke, K. M. Enhanced pathological angiogenesis in mice lacking beta-3 integrin or beta-3 and beta-5 integrins. Nature Med. 8: 27-34, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Integrin beta 5

Target: Integrin beta 5 (ITGB5)

Alternative Name: ITGB5

Background:

Synonyms: Heat shock protein 105 kDa, Antigen NY-CO-25, Heat shock 110 kDa protein, HSPH1, HSP105, HSP110, KIAA0201

Tissue Specificity: Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

Background: This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin.

Molecular Weight: 100 kDa

Gene ID: 3693

UniProt: P18084

Pathways: Integrin Complex