CD73 antibody (AA 337-551)

Catalog No. ABIN7601367

This Rabbit Polyclonal antibody specifically detects CD73 in WB, IHC, FACS and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for CD73 antibody (AA 337-551) (ABIN7601367)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Product Details anti-CD73 Antibody

Binding Specificity: AA 337-551

Purpose: Anti-CD73/Nt5e Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD73/Nt5e Antibody Picoband® (ABIN7601367). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD73/Nt5e recombinant protein (Position: S337-S551).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Boyle, J. M., Hey, Y., Fox, M. Human ecto-5-prime nucleotidase maps to chromosome 6q14-q21. (Abstract) Cytogenet. Cell Genet. 51: 968 only, 1989. 2. Castrop, H., Huang, Y., Hashimoto, S., Mizel, D., Hansen, P., Theilig, F., Bachmann, S., Deng, C., Briggs, J., Schnermann, J. Impairment of tubuloglomerular feedback regulation of GFR in ecto-5-prime-nucleotidase/CD73-deficient mice. J. Clin. Invest. 114: 634-642, 2004. 3. St. Hilaire, C., Ziegler, S. G., Markello, T. C., Brusco, A., Groden, C., Gill, F., Carlson-Donohoe, H., Lederman, R. J., Chen, M. Y., Yang, D., Siegenthaler, M. P., Arduino, C., and 9 others. NT5E mutations and arterial calcifications. New Eng. J. Med. 364: 432-442, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: Nt5e

Background:

Synonyms: Carnitine O-palmitoyltransferase 2, mitochondrial, Carnitine palmitoyltransferase II, CPT II, CPT2, CPT1

Tissue Specificity: Expressed mainly in adipose tissues.

Background: The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 63 kDa

Gene ID: 23959

UniProt: Q61503

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process