CLPX antibody (AA 337-574)

Catalog No. ABIN7601368

The Mouse Monoclonal anti-CLPX antibody has been validated for WB, IF and ICC. It is suitable to detect CLPX in samples from Human.

Quick Overview for CLPX antibody (AA 337-574) (ABIN7601368)

Target: CLPX (ClpX Caseinolytic Peptidase X (CLPX))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLPX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 11C11

Product Details anti-CLPX Antibody

Binding Specificity: AA 337-574

Purpose: Anti-CLPX Antibody Picoband® (monoclonal, 11C11)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CLPX Antibody Picoband® (monoclonal, 11C11) (ABIN7601368). Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human CLPX recombinant protein (Position: A337-E574).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
1. Corydon, T. J., Wilsbech, M., Jespersgaard, C., Andresen, B. S., Borglum, A. D., Pedersen, S., Bolund, L., Gregersen, N., Bross, P. Human and mouse mitochondrial orthologs of bacterialClpX. Mammalian Genome 11: 899-905, 2000. 2. Kang, S. G., Dimitrova, M. N., Ortega, J., Ginsburg, A., Maurizi, M. R. Human mitochondrial ClpP is a stable heptamer that assembles into a tetradecamer in the presence of ClpX. J. Biol. Chem. 280: 35424-35432, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.01 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CLPX

Target: CLPX (ClpX Caseinolytic Peptidase X (CLPX))

Alternative Name: CLPX

Background:

Synonyms: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial, CLPX

Tissue Specificity: Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.

Background: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial is an enzyme that in humans is encoded by the CLPX gene. This protein is a member of the family of AAA Proteins (AAA+ ATPase) and is to form the protein complex of Clp protease. The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.

Molecular Weight: 69 kDa

Gene ID: 10845

UniProt: O76031