DAZL antibody (AA 34-281)

Catalog No. ABIN7601384

This anti-DAZL antibody is a Rabbit Polyclonal antibody detecting DAZL in WB, ELISA, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for DAZL antibody (AA 34-281) (ABIN7601384)

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAZL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-DAZL Antibody

Binding Specificity: AA 34-281

Purpose: Anti-DAZL Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DAZL Picoband® Antibody (ABIN7601384). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DAZL recombinant protein (Position: E34-R281).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Bartoloni, L., Cazzadore, C., Ferlin, A., Garolla, A., Foresta, C. Lack of the T54A polymorphism of the DAZL gene in infertile Italian patients. Molec. Hum. Reprod. 10: 613-615, 2004. 2. Eberhart, C. G., Maines, J. Z., Wasserman, S. A. Meiotic cell cycle requirement for a fly homologue of human Deleted in Azoospermia. Nature 381: 783-785, 1996. 3. Kee, K., Angeles, V. T., Flores, M., Nguyen, H. N., Reijo Pera, R. A. Human DAZL, DAZ and BOULE genes modulate primordial germ-cell and haploid gamete formation. Nature 462: 222-225, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DAZL

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Alternative Name: DAZL

Background:

Synonyms: Deleted in azoospermia-like, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1, SPGY-like-autosomal, DAZL, DAZH, DAZL1, DAZLA, SPGYLA

Background: Deleted in azoospermia-like is a protein that in humans is encoded by the DAZL gene. It is mapped to 3p24.3. The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 38 kDa

Gene ID: 1618

UniProt: Q92904