HIBADH antibody (AA 34-332)

Catalog No. ABIN7601387

This anti-HIBADH antibody is a Rabbit Polyclonal antibody detecting HIBADH in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for HIBADH antibody (AA 34-332) (ABIN7601387)

Target: HIBADH (3-hydroxyisobutyrate Dehydrogenase (HIBADH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIBADH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-HIBADH Antibody

Binding Specificity: AA 34-332

Purpose: Anti-HIBADH Antibody Picoband®

Characteristics: Anti-HIBADH Antibody Picoband® (ABIN7601387). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HIBADH recombinant protein (Position: R34-E332). Human HIBADH shares 93.3% and 92.6% amino acid (aa) sequence identity with mouse and rat HIBADH, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Rougraff, P. M., Zhang, B., Kuntz, M. J., Harris, R. A., Crabb, D. W. Cloning and sequence analysis of a cDNA for 3-hydroxyisobutyrate dehydrogenase: evidence for its evolutionary relationship to other pyridine nucleotide-dependent dehydrogenases. J. Biol. Chem. 264: 5899-5903, 1989.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HIBADH

Target: HIBADH (3-hydroxyisobutyrate Dehydrogenase (HIBADH))

Alternative Name: HIBADH

Background: In enzymology, a 3-hydroxyisobutyrate dehydrogenase also known as β-hydroxyisobutyrate dehydrogenase or 3-hydroxyisobutyrate dehydrogenase, mitochondrial (HIBADH) is an enzyme that in humans is encoded by the HIBADH gene. This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde.

Molecular Weight: 32 kDa

Gene ID: 11112

UniProt: P31937