OXCT1 antibody (AA 35-309)

Catalog No. ABIN7601419

The Rabbit Polyclonal anti-OXCT1 antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect OXCT1 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for OXCT1 antibody (AA 35-309) (ABIN7601419)

Target: OXCT1 (3-Oxoacid CoA Transferase 1 (OXCT1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OXCT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-OXCT1 Antibody

Binding Specificity: AA 35-309

Purpose: Anti-OXCT1/SCOT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-OXCT1/SCOT Antibody Picoband® (ABIN7601419). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human OXCT1/SCOT recombinant protein (Position: S35-A309).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fukao, T., Mitchell, G. A., Song, X.-Q., Nakamura, H., Kassovska-Bratinova, S., Orii, K. E., Wraith, J. E., Besley, G., Wanders, R. J. A., Niezen-Koning, K. E., Berry, G. T., Palmieri, M., Kondo, N. Succinyl-CoA:3-ketoacid CoA transferase (SCOT): cloning of the human SCOT gene, tertiary structural modeling of the human SCOT monomer, and characterization of three pathogenic mutations. Genomics 68: 144-151, 2000. 2. Hori, T., Fukao, T., Murase, K., Sakaguchi, N., Harding, C. O., Kondo, N. Molecular basis of two-exon skipping (exons 12 and 13) by c.1248+5g-a in OXCT1 gene: study on intermediates of OXCT1 transcripts in fibroblasts. Hum. Mutat. 34: 473-480, 2013. 3. Kassovska-Bratinova, S., Fukao, T., Song, X.-Q., Duncan, A. M. V., Chen, H. S., Robert, M.-F., Perez-Cerda, C., Ugarte, M., Chartrand, C., Vobecky, S., Kondo, N., Mitchell, G. A. Succinyl CoA:3-oxoacid CoA transferase (SCOT): human cDNA cloning, human chromosomal mapping to 5p13, and mutation detection in a SCOT-deficient patient. Am. J. Hum. Genet. 59: 519-528, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for OXCT1

Target: OXCT1 (3-Oxoacid CoA Transferase 1 (OXCT1))

Alternative Name: OXCT1

Background:

Synonyms: DNA- (apurinic or apyrimidinic site) lyase 2,3.1.-.-,4.2.99.18,AP endonuclease XTH2,APEX nuclease 2,APEX nuclease-like 2,Apurinic-apyrimidinic endonuclease 2,AP endonuclease 2,APEX2,APE2, APEXL2, XTH2,

Tissue Specificity: Highly expressed in brain and kidney. Weakly expressed in the fetal brain. .

Background: 3-oxoacid CoA-transferase 1 (OXCT1) is an enzyme that in humans is encoded by the OXCT1 gene. It is also known as succinyl-CoA-3-oxaloacid CoA transferase (SCOT). This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.

Molecular Weight: 56 kDa

Gene ID: 5019

UniProt: P55809

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis