CD55 antibody (AA 35-347)

Catalog No. ABIN7601422

The Mouse Monoclonal anti-CD55 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect CD55 in samples from Human.

Quick Overview for CD55 antibody (AA 35-347) (ABIN7601422)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 5B9E1

Product Details anti-CD55 Antibody

Binding Specificity: AA 35-347

Purpose: Anti-CD55 Antibody Picoband® (monoclonal, 5B9E1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD55 Antibody Picoband® (monoclonal, 5B9E1) (ABIN7601422). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD55 recombinant protein (Position: D35-K347). Human CD55 shares 49.1% amino acid (aa) sequence identity with mouse CD55.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
1. Medof ME, Lublin DM, Holers VM, Ayers DJ, Getty RR, Leykam JF, Atkinson JP, Tykocinski ML (April 1987). "Cloning and characterization of cDNAs encoding the complete sequence of decay-accelerating factor of human complement"". Proc. Natl. Acad. Sci. U.S.A. 84 (7): 2007-11.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55

Background:

Synonyms: ATP dependent helicase RENT1 antibody, ATP-dependent helicase RENT1 antibody, Delta helicase antibody, FLJ43809 antibody, FLJ46894 antibody, HUPF 1 antibody, hUpf1 antibody, KIAA0221 antibody, Nonsense mRNA reducing factor 1 antibody, NORF 1 antibody, NORF1 antibody, pNORF 1 antibody, pNORF1 antibody, Regulator of nonsense transcripts 1 antibody, RENT 1 antibody, RENT1 antibody, RENT1_HUMAN antibody, Smg 2 antibody, Smg 2 homolog nonsense mediated mRNA decay factor antibody, UP Frameshift 1 antibody, Up frameshift mutation 1 homolog (S. cerevisiae) antibody, Up frameshift mutation 1 homolog antibody, Up frameshift suppressor 1 homolog antibody, Up-frameshift suppressor 1 homolog antibody, UPF 1 antibody, UPF 1 regulator of nonsense transcripts homolog antibody, upf1 antibody, UPF1 regulator of nonsense transcripts homolog antibody, Yeast Upf1p homolog antibody

Tissue Specificity: Ubiquitous.

Background: Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.

Molecular Weight: 70-75 kDa

Gene ID: 1604

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity