LGI1 antibody (AA 35-557)

Catalog No. ABIN7601430

This Rabbit Polyclonal antibody specifically detects LGI1 in WB, ELISA and FACS. It exhibits reactivity toward Mouse, Human and Rat.

Quick Overview for LGI1 antibody (AA 35-557) (ABIN7601430)

Target: LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-LGI1 Antibody

Binding Specificity: AA 35-557

Purpose: Anti-Lgi1/EPT/LGI1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Lgi1/EPT/LGI1 Antibody Picoband® (ABIN7601430). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Lgi1/EPT/LGI1 recombinant protein (Position: K35-A557).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Anderson, M. P. Arrested glutamatergic synapse development in human partial epilepsy. Epilepsy Curr. 10: 153-158, 2010. 2. Bigner, S. H., Vogelstein, B. Cytogenetics and molecular genetics of malignant gliomas and medulloblastoma. Brain Path. 1: 12-18, 1990. 3. Bisulli, F., Tinuper, P., Scudellaro, E., Naldi, I., Bagattin, A., Avoni, P., Michelucci, R., Nobile, C. A de novo LGI1 mutation in sporadic partial epilepsy with auditory features. Ann. Neurol. 56: 455-456, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for LGI1

Target: LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

Alternative Name: LGI1

Background:

Synonyms: Complement component C7, C7

Tissue Specificity: Widely expressed.

Background: Leucine-rich, glioma inactivated 1, also known as LGI1, is a protein which in humans is encoded by the LGI1 gene. This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants.

Molecular Weight: 64 kDa

Gene ID: 9211

UniProt: O95970