ZW10 antibody (AA 35-779)

Catalog No. ABIN7601437

The Rabbit Polyclonal anti-ZW10 antibody has been validated for WB, ELISA and FACS. It is suitable to detect ZW10 in samples from Human.

Quick Overview for ZW10 antibody (AA 35-779) (ABIN7601437)

Target: ZW10 (Zw10 Kinetochore Protein (ZW10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZW10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ZW10 Antibody

Binding Specificity: AA 35-779

Purpose: Anti-ZW10 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ZW10 Antibody Picoband® (ABIN7601437). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ZW10 recombinant protein (Position: K35-K779).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chan, G. K. T., Jablonski, S. A., Starr, D. A., Goldberg, M. L., Yen, T. J. Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores. Nature Cell Biol. 2: 944-947, 2000. 2. Musio, A., Mariani, T., Montagna, C., Zambroni, D., Ascoli, C., Ried, T., Vezzoni, P. Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes. Gene 331: 33-40, 2004. 3. Scaerou, F., Starr, D. A., Piano, F., Papoulas, O., Karess, R. E., Goldberg, M. L. The ZW10 and rough deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore. J. Cell Sci. 114: 3103-3114, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ZW10

Target: ZW10 (Zw10 Kinetochore Protein (ZW10))

Alternative Name: ZW10

Background:

Synonyms: Calretinin, CR, 29 kDa calbindin, CALB2, CAB29

Tissue Specificity: Brain.

Background: Centromere/kinetochore protein zw10 homolog is a protein that in humans is encoded by the ZW10 gene This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis.

Molecular Weight: 95 kDa

Gene ID: 9183

UniProt: O43264