DMC1 antibody (AA 36-283)

Catalog No. ABIN7601468

This anti-DMC1 antibody is a Rabbit Polyclonal antibody detecting DMC1 in WB, ELISA, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for DMC1 antibody (AA 36-283) (ABIN7601468)

Target: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DMC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-DMC1 Antibody

Binding Specificity: AA 36-283

Purpose: Anti-DMC1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DMC1 Antibody Picoband® (ABIN7601468). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DMC1 recombinant protein (Position: D36-D283).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cloud, V., Chan, Y.-L., Grubb, J., Budke, B., Bishop, D. K. Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis. Science 337: 1222-1225, 2012. 2. Habu, T., Taki, T., West, A., Nishimune, Y., Morita, T. The mouse and human homologs of DMC1, the yeast meiosis-specific homologous recombination gene, have a common unique form of exon-skipped transcript in meiosis. Nucleic Acids Res. 24: 470-477, 1996. 3. He, W.-B., Tu, C.-F., Liu, Q., Meng, L.-L., Yuan, S.-M., Luo, A.-X., He, F.-S., Shen, J., Li, W., Du, J., Zhong, C.-G., Lu, G.-X., Lin, G., Fan, L.-Q., Tan, Y.-Q. DMC1 mutation that causes human non-obstructive azoospermia and premature ovarian insufficiency identified by whole-exome sequencing. J. Med. Genet. 55: 198-204, 2018.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DMC1

Target: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Alternative Name: DMC1

Background:

Synonyms: Aldo-keto reductase family 1 member B10,1.1.1.-,ARL-1,Aldose reductase-like,Aldose reductase-related protein,ARP,hARP,Small intestine reductase,SI reductase,AKR1B10,AKR1B11,

Tissue Specificity: Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.

Background: Meiotic recombination protein DMC1/LIM15 homolog is a protein that in humans is encoded by the DMC1 gene. This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. Alternative splicing results in multiple transcript variants.

Molecular Weight: 37 kDa

Gene ID: 11144

UniProt: Q14565