PRAM1 antibody (AA 361-670)

Catalog No. ABIN7601484

This Rabbit Polyclonal antibody specifically detects PRAM1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for PRAM1 antibody (AA 361-670) (ABIN7601484)

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PRAM1 Antibody

Binding Specificity: AA 361-670

Purpose: Anti-PRAM1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PRAM1 Antibody Picoband® (ABIN7601484). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRAM1 recombinant protein (Position: E361-R670).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Moog-Lutz, C., Peterson, E. J., Lutz, P. G., Eliason, S., Cave-Riant, F., Singer, A., Di Gioia, Y., Dmowski, S., Kamens, J., Cayre, Y. E., Koretzky, G. PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells. J. Biol. Chem. 276: 22375-22381, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PRAM1

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Alternative Name: PRAM1

Background:

Synonyms: Protein NDRG3,N-myc downstream-regulated gene 3 protein,NDRG3,

Tissue Specificity: Ubiquitous. Highly expressed in brain. .

Background: PML-RARA-regulated adapter molecule 1 is a protein that in humans is encoded by the PRAM1 gene. The protein encoded by this gene is similar to FYN binding protein (FYB/SLAP-130), an adaptor protein involved in T cell receptor mediated signaling. This gene is expressed and regulated during normal myelopoiesis. The expression of this gene is induced by retinoic acid and is inhibited by the expression of PML-RARalpha, a fusion protein of promyelocytic leukemia (PML) and the retinoic acid receptor-alpha (RARalpha).

Molecular Weight: 90 kDa

Gene ID: 84106

Pathways: Regulation of Leukocyte Mediated Immunity