KIF5A antibody (AA 374-1032)

Catalog No. ABIN7601527

The Rabbit Polyclonal anti-KIF5A antibody has been validated for WB, ELISA and FACS. It is suitable to detect KIF5A in samples from Human, Mouse and Rat.

Quick Overview for KIF5A antibody (AA 374-1032) (ABIN7601527)

Target: KIF5A (Kinesin Family Member 5A (KIF5A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-KIF5A Antibody

Binding Specificity: AA 374-1032

Purpose: Anti-KIF5A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KIF5A Antibody Picoband® (ABIN7601527). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KIF5A recombinant protein (Position: E374-S1032).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Blair, M. A., Ma, S., Hedera, P. Mutation in KIF5A can also cause adult-onset hereditary spastic paraplegia. Neurogenetics 7: 47-50, 2006. 2. Brenner, D., Yilmaz, R., Muller, K., Grehl, T., Petri, S., Meyer, T., Grosskreutz, J., Weydt, P., Ruf, W., Neuwirth, C., Weber, M., Pinto, S., and 29 others. Hot-spot KIF5A mutations cause familial ALS. Brain 141: 688-697, 2018. 3. Crimella, C., Baschirotto, C., Arnoldi, A., Tonelli, A., Tenderini, E., Airoldi, G., Martinuzzi, A., Trabacca, A., Losito, L., Scarlato, M., Benedetti, S., Scarpini, E., Spinicci, G., Bresolin, N., Bassi, M. T. Mutations in the motor and stalk domains of KIF5A in spastic paraplegia type 10 and in axonal Charcot-Marie-Tooth type 2. Clin. Genet. 82: 157-164, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for KIF5A

Target: KIF5A (Kinesin Family Member 5A (KIF5A))

Alternative Name: KIF5A

Background:

Synonyms: Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, LMAN1, ERGIC53, F5F8D

Tissue Specificity: Ubiquitous.

Background: Kinesin heavy chain isoform 5A is a protein that in humans is encoded by the KIF5A gene. This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10.

Molecular Weight: 117 kDa

Gene ID: 3798

UniProt: Q12840