HGD antibody (AA 374-445)

Catalog No. ABIN7601528

This anti-HGD antibody is a Mouse Monoclonal antibody detecting HGD in WB, IHC and FACS. Suitable for Human.

Quick Overview for HGD antibody (AA 374-445) (ABIN7601528)

Target: HGD (Homogentisate 1,2-Dioxygenase (HGD))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HGD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 2F11E1

Product Details anti-HGD Antibody

Binding Specificity: AA 374-445

Purpose: Anti-HGD Antibody Picoband® (monoclonal, 2F11E1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HGD Antibody Picoband® (monoclonal, 2F11E1) (ABIN7601528). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human HGD recombinant protein (Position: D374-N445).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Fernandez-Canon, J. M., Granadino, B., Beltran-Valero de Bernabe, D., Renedo, M., Fernandez-Ruiz, E., Penalva, M. A., Rodriguez de Cordoba, S. The molecular basis of alkaptonuria. Nature Genet. 14: 19-24, 1996. 2. Vilboux, T., Kayser, M., Introne, W., Suwannarat, P., Bernardini, I., Fischer, R., O'Brien, K., Kleta, R., Huizing, M., Gahl, W. A. Mutation spectrum of homogentisic acid oxidase (HGD) in alkaptonuria. Hum. Mutat. 30: 1611-1619, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HGD

Target: HGD (Homogentisate 1,2-Dioxygenase (HGD))

Alternative Name: HGD

Background:

Synonyms: Ras-related protein Rab-5A, RAB5A, RAB5

Tissue Specificity: Widely expressed in fetal and adult tissues.

Background: The HGD gene encodes homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the catabolism of phenylalanine and tyrosine. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria. This gene is mapped to chromosome 3q21-q23 by a preliminary PCR screen of hamster/human somatic cell hybrid genomic DNA samples and by fluorescence in situ hybridization.

Molecular Weight: 50 kDa

Gene ID: 3081

UniProt: Q93099