Myosin VI antibody (AA 383-786)

Catalog No. ABIN7601557

The Rabbit Polyclonal anti-Myosin VI antibody has been validated for WB, ELISA and FACS. It is suitable to detect Myosin VI in samples from Human and Mouse.

Quick Overview for Myosin VI antibody (AA 383-786) (ABIN7601557)

Target: Myosin VI (MYO6)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myosin VI antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Myosin VI Antibody

Binding Specificity: AA 383-786

Purpose: Anti-MYO6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MYO6 Antibody Picoband® (ABIN7601557). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MYO6 recombinant protein (Position: Q383-H786).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ahituv, N., Sobe, T., Robertson, N. G., Morton, C. C., Taggart, R. T., Avraham, K. B. Genomic structure of the human unconventional myosin VI gene. Gene 261: 269-275, 2000. 2. Ahmed, Z. M., Morell, R. J., Riazuddin, S., Gropman, A., Shaukat, S., Ahmad, M. M., Mohiddin, S. A., Fananapazir, L., Caruso, R. C., Husnain, T., Khan, S. N., Riazuddin, S., Griffith, A. J., Friedman, T. B., Wilcox, E. R. Mutations of MYO6 are associated with recessive deafness, DFNB37. Am. J. Hum. Genet. 72: 1315-1322, 2003. 3. Altman, D., Sweeney, H. L., Spudich, J. A. The mechanism of myosin VI translocation and its load-induced anchoring. Cell 116: 737-749, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Myosin VI

Target: Myosin VI (MYO6)

Alternative Name: MYO6

Background:

Synonyms: Homeobox protein SIX3,Sine oculis homeobox homolog 3,SIX3,

Tissue Specificity: Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.

Background: Unconventional myosin-VI, is a protein that in humans is coded for by MYO6. This gene encodes a reverse-ion motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The protein consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 150 kDa

Gene ID: 4646

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport, Asymmetric Protein Localization