AKAP12 antibody (AA 387-1620)

Catalog No. ABIN7601563

The Rabbit Polyclonal anti-AKAP12 antibody has been validated for WB, IHC, ELISA, IF, ICC and FACS. It is suitable to detect AKAP12 in samples from Rat and Mouse.

Quick Overview for AKAP12 antibody (AA 387-1620) (ABIN7601563)

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-AKAP12 Antibody

Binding Specificity: AA 387-1620

Purpose: Anti-AKAP12 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AKAP12 Antibody Picoband® (ABIN7601563). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse AKAP12 recombinant protein (Position: E387-H1620).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Gordon, T., Grove, B., Loftus, J. C., O'Toole, T., McMillan, R., Lindstrom, J., Ginsberg, M. H. Molecular cloning and preliminary characterization of a novel cytoplasmic antigen recognized by myasthenia gravis sera. J. Clin. Invest. 90: 992-999, 1992. 2. Lee, S.-W., Kim, W. J., Choi, Y. K., Song, H. S., Son, M. J., Gelman, I. H., Kim, Y.-J., Kim, K.-W. SSeCKS regulates angiogenesis and tight junction formation in blood-brain barrier. Nature Med. 9: 900-906, 2003. 3. Nauert, J. B., Klauck, T. M., Langeberg, L. K., Scott, J. D. Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein. Curr. Biol. 7: 52-62, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AKAP12

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Alternative Name: Akap12

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 14,Herpes virus entry mediator A,Herpesvirus entry mediator A,HveA,Tumor necrosis factor receptor-like 2,TR2,CD270,TNFRSF14,HVEA, HVEM,UNQ329/PRO509,

Tissue Specificity: Widely expressed, with the highest expression in lung, spleen and thymus.

Background: A-kinase anchor protein 12, aka AKAP250, is an enzyme that in humans is encoded by the AKAP12 gene. The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinase A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Molecular Weight: 300 kDa

Gene ID: 83397

Pathways: cAMP Metabolic Process