NDST4 antibody (AA 39-260)

Catalog No. ABIN7601571

This Rabbit Polyclonal antibody specifically detects NDST4 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for NDST4 antibody (AA 39-260) (ABIN7601571)

Target: NDST4 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 4 (NDST4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDST4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NDST4 Antibody

Binding Specificity: AA 39-260

Purpose: Anti-NDST4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NDST4 Antibody Picoband® (ABIN7601571). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NDST4 recombinant protein (Position: Q39-L260).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aikawa, J., Grobe, K., Tsujimoto, M., Esko, J. D. Multiple isozymes of heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase: structure and activity of the fourth member, NDST4. J. Biol. Chem. 276: 5876-5882, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NDST4

Target: NDST4 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 4 (NDST4))

Alternative Name: NDST4

Background:

Synonyms: Protein eva-1 homolog A, Protein FAM176A, Transmembrane protein 166, EVA1A, FAM176A, TMEM166, SP24

Tissue Specificity: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.

Background: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity (By similarity).

Molecular Weight: 101 kDa

Gene ID: 64579

UniProt: Q9H3R1

Pathways: Glycosaminoglycan Metabolic Process