ACP5 antibody (AA 39-325)

Catalog No. ABIN7601574

This Rabbit Polyclonal antibody specifically detects ACP5 in WB, IHC, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ACP5 antibody (AA 39-325) (ABIN7601574)

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-ACP5 Antibody

Binding Specificity: AA 39-325

Purpose: Anti-Acid phosphatase/ACP5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Acid phosphatase/ACP5 Antibody Picoband® (ABIN7601574). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Acid phosphatase/ACP5 recombinant protein (Position: N39-P325).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Allen, B. S., Ketcham, C. M., Roberts, R. M., Nick, H. S., Ostrer, H. Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization. Genomics 4: 597-600, 1989. 2. Bilginer, Y., Duzova, A., Topaloglu, R., Batu, E. D., Boduroglu, K., Gucer, S., Bodur, I., Alanay, Y. Three cases of spondyloenchondrodysplasia (SPENCD) with systemic lupus erythematosus: a case series and review of the literature. Lupus 25: 760-765, 2016. 3. Briggs, T. A., Rice, G. I., Adib, N., Ades, L., Barete, S., Baskar, K., Baudouin, V., Cebeci, A. N., Clapuyt, P., Coman, D., De Somer, L., Finezilber, Y., and 19 others. Spondyloenchondrodysplasia due to mutations in ACP5: a comprehensive survey. J. Clin. Immun. 36: 220-234, 2016. Note: Erratum: J. Clin. Immun. 36: 529-530, 2016.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ACP5

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Alternative Name: ACP5

Background:

Synonyms: Leucine-rich PPR motif-containing protein, mitochondrial, 130 kDa leucine-rich protein, LRP 130, GP130, LRPPRC, LRP130

Tissue Specificity: Expressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes.

Background: Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5), is a glycosylated monomeric metalloprotein enzyme expressed in mammals. This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.

Molecular Weight: 39 kDa

Gene ID: 54

UniProt: P13686

Pathways: Transition Metal Ion Homeostasis