ANXA6 antibody (AA 395-665)

Catalog No. ABIN7601597

This anti-ANXA6 antibody is a Mouse Monoclonal antibody detecting ANXA6 in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for ANXA6 antibody (AA 395-665) (ABIN7601597)

Target: ANXA6 (Annexin A6 (ANXA6))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANXA6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 4C4

Product Details anti-ANXA6 Antibody

Binding Specificity: AA 395-665

Purpose: Anti-Annexin VI Antibody (monoclonal, 4C4)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Annexin VI Antibody (monoclonal, 4C4) (ABIN7601597). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Annexin VI recombinant protein (Position: N395-L665).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ANXA6

Target: ANXA6 (Annexin A6 (ANXA6))

Alternative Name: ANXA6

Background:

Synonyms: Eukaryotic translation initiation factor 6, eIF-6, B (2)GCN homolog, B4 integrin interactor, CAB, p27 (BBP), EIF6, EIF3A, ITGB4BP, OK/SW-cl.27

Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle.

Background: Annexin A6 (ANXA6) is a member of a family of proteins that bind membrane or cytoskeleton in a Ca(2+)-dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. ANXA6 gene is assigned to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization. The ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal. Ca(2+)-dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion.

Molecular Weight: 72 kDa

Gene ID: 309

UniProt: P08133