CAPNL1 antibody (AA 396-555)

Catalog No. ABIN7601599

The Mouse Monoclonal anti-CAPNL1 antibody has been validated for WB and FACS. It is suitable to detect CAPNL1 in samples from Human.

Quick Overview for CAPNL1 antibody (AA 396-555) (ABIN7601599)

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAPNL1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 2E3

Product Details anti-CAPNL1 Antibody

Binding Specificity: AA 396-555

Purpose: Anti-Calpain 1 Antibody Picoband® (monoclonal, 2E3)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Calpain 1 Antibody Picoband® (monoclonal, 2E3) (ABIN7601599). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Smith IJ, et al. Calpain activity is increased in skeletal muscle from gastric cancer patients with no or minimal weight loss. Muscle Nerve, 2011 Mar. 2. Wang JC, et al. Proteolysis by endogenous calpain I leads to the activation of calcineurin in human heart. Clin Lab, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CAPNL1

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Alternative Name: CAPN1

Background:

Synonyms: Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan, Bamacan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, hCAP, SMC3, BAM, BMH, CSPG6, SMC3L1

Tissue Specificity: Ubiquitous. Expressed in the rod and cone photoreceptors, extending from the tips of the outer segment (OS) through the inner segment (IS) and outer nuclear layer (ONL) and into the synaptic terminals of the outer plexiform layer (ONL). Also detected in the bipolar, horizontal and amacrine cells in the inner nuclear layer (INL), extending to the inner plexiform layer (IPL) and though the ganglion cell layer (GCL) and into the nerve fiber layer (NFL) (at protein level).

Background: CAPN1 is also known as CANP or muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

Molecular Weight: 82 kDa

Gene ID: 823

UniProt: P07384

Pathways: Apoptosis