SPATA20 antibody (AA 396-786)

Catalog No. ABIN7601605

Product Details anti-SPATA20 Antibody

Target: SPATA20 (Spermatogenesis Associated 20 (SPATA20))

Binding Specificity: AA 396-786

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPATA20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Anti-SPATA20 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SPATA20 Antibody Picoband® (ABIN7601605). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPATA20 recombinant protein (Position: K396-P786).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 4/19/2011. 2. Shi, H.-J., Wu, A. Z., Santos, M., Feng, Z.-M., Huang, L., Chen, Y.-M., Zhu, K., Chen, C.-L. C. Cloning and characterization of rat spermatid protein SSP411: a thioredoxin-like protein. J. Androl. 25: 479-493, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SPATA20

Target: SPATA20 (Spermatogenesis Associated 20 (SPATA20))

Alternative Name: SPATA20 (SPATA20 Products)

Background:

Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: SPATA20 (Spermatogenesis Associated 20), located on human chromosome 17q21.33, is a member of thioredoxin family. The human SPATA20 gene, also known as SSP411, Tisp78 and HEL-S-98, encodes an 87899 Da protein containing 786 amino acids. The SPATA20 protein is broadly expressed in testis, thyroid and other tissues. The SPATA20 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, etc. 336 organisms have orthologs with human gene SPATA20. SPATA20 is associated with some diseases, including Common Bile Duct Disease and Choledocholithiasis.

Molecular Weight: 88 kDa

Gene ID: 64847