GCLM antibody (AA 4-263)

Catalog No. ABIN7601616

This Rabbit Polyclonal antibody specifically detects GCLM in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GCLM antibody (AA 4-263) (ABIN7601616)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GCLM Antibody

Binding Specificity: AA 4-263

Purpose: Anti-GCLM Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GCLM Antibody Picoband® (ABIN7601616). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GCLM recombinant protein (Position: D4-K263).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Arinami, T., Ohtsuki, T., Ishiguro, H., Ujike, H., Tanaka, Y., Morita, Y., Mineta, M., Takeichi, M., Yamada, S., Imamura, A., Ohara, K., Shibuya, H., and 40 others. Genomewide high-density SNP linkage analysis of 236 Japanese families supports the existence of schizophrenia susceptibility loci on chromosomes 1p, 14q, and 20p. Am. J. Hum. Genet. 77: 937-944, 2005. 2. Gipp, J. J., Bailey, H. H., Mulcahy, R. T. Cloning and sequencing of the cDNA for the light subunit of human liver gamma-glutamylcysteine synthetase and relative mRNA levels for heavy and light subunits in human normal tissues. Biochem. Biophys. Res. Commun. 206: 584-589, 1995. 3. Huang, C.-S., Anderson, M. E., Meister, A. Amino acid sequence and function of the light subunit of rat kidney gamma-glutamylcysteine synthetase. J. Biol. Chem. 268: 20578-20583, 1993.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name: GCLM

Background:

Synonyms: Caspase-4, CASP-4

Tissue Specificity: Widely expressed, including in thymus, lung and spleen (at protein level). Very low levels, if any, in the brain.

Background: Glutamate-cysteine ligase regulatory subunit is an enzyme that in humans is encoded by the GCLM gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 31 kDa

Gene ID: 2730

UniProt: P48507