Replication Termination Factor 2 (RTF2) (AA 4-306) antibody

Catalog No. ABIN7601620

The Rabbit Polyclonal anti- antibody has been validated for ELISA, IP, WB, FACS, ICC and IF. It is suitable to detect in samples from Human.

Quick Overview for Replication Termination Factor 2 (RTF2) (AA 4-306) antibody (ABIN7601620)

Target: Replication Termination Factor 2 (RTF2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 4-306

Purpose: Anti-RTF2 Antibody Picoband®

Characteristics: Anti-RTF2 Antibody Picoband® (ABIN7601620). Tested in ELISA, Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RTF2 recombinant protein (Position: D4-F306). Human RTF2 shares 87.8% and 88.1% amino acid (aa) sequence identity with mouse and rat RTF2, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chia, B. S., Li, B., Cui, A., Eisenhaure, T., Raychowdhury, R., Lieb, D., & Hacohen, N. (2020). Loss of the nuclear protein RTF2 enhances influenza virus replication. Journal of Virology, 94(22), 10-1128. 2. Suo, D., Wang, L., Zeng, T., Zhang, H., Li, L., Liu, J., ... & Li, Y. (2020). NRIP3 upregulation confers resistance to chemoradiotherapy in ESCC via RTF2 removal by accelerating ubiquitination and degradation of RTF2. Oncogenesis, 9(8), 75. 3. Kottemann, M. C., Conti, B. A., Lach, F. P., & Smogorzewska, A. (2018). Removal of RTF2 from stalled replisomes promotes maintenance of genome integrity. Molecular cell, 69(1), 24-35.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Replication Termination Factor 2 (RTF2)

Alternative Name: RTF2

Background: Replication termination factor 2 is a protein that in humans is encoded by the RTF2 gene. Replication Termination Factor 2, often abbreviated as RTF2, is a protein involved in the regulation and termination of DNA replication processes in eukaryotic cells. It plays a crucial role in ensuring the accurate completion of DNA synthesis during cell division by coordinating the termination of replication forks at specific sites along the DNA strands. RTF2 functions by interacting with other proteins and DNA sequences to facilitate the orderly disassembly of the replication machinery and the prevention of unnecessary re-replication of genomic regions. Its activity is tightly regulated to maintain genomic stability and fidelity, making it a key player in cell cycle control mechanisms. Studies on RTF2 have provided insights into the molecular mechanisms governing DNA replication termination and its implications for cellular function and genome maintenance.

Molecular Weight: 37 kDa

Gene ID: 51507