NOS2 antibody (AA 40-1099)

Catalog No. ABIN7601635

The Rabbit Polyclonal anti-NOS2 antibody has been validated for WB, ELISA and FACS. It is suitable to detect NOS2 in samples from Mouse.

Quick Overview for NOS2 antibody (AA 40-1099) (ABIN7601635)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NOS2 Antibody

Binding Specificity: AA 40-1099

Purpose: Anti-iNOS/Nos2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-iNOS/Nos2 Antibody Picoband® (ABIN7601635). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse iNOS/Nos2 recombinant protein (Position: I40-L1099).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Chartrain, N. A., Geller, D. A., Koty, P. P., Sitrin, N. F., Nussler, A. K., Hoffman, E. P., Billiar, T. R., Hutchinson, N. I., Mudgett, J. S. Molecular cloning, structure, and chromosomal localization of the human inducible nitric oxide synthase gene. J. Biol. Chem. 269: 6765-6772, 1994. 2. Diefenbach, A., Schindler, H., Rollinghoff, M., Yokoyama, W. M., Bogdan, C. Requirement for type 2 NO synthase for IL-12 signaling in innate immunity. Science 284: 951-955, 1999. 3. Hobbs, M. R., Udhayakumar, V., Levesque, M. C., Booth, J., Roberts, J. M., Tkachuk, A. N., Pole, A., Coon, H., Kariuki, S., Nahlen, B. L., Mwaikambo, E. D., Lai, A. L., Granger, D. L., Anstey, N. M., Weinberg, J. B. A new NOS2 promoter polymorphism associated with increased nitric oxide production and protection from severe malaria in Tanzanian and Kenyan children. Lancet 360: 1468-1475, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: Nos2

Background:

Synonyms: Nitric oxide synthase, inducible,1.14.13.39,Hepatocyte NOS,HEP-NOS,Inducible NO synthase,Inducible NOS,iNOS,N

Tissue Specificity: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

Background: Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter, it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity.

Molecular Weight: 130 kDa

Gene ID: 18126

UniProt: P29477

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process