NSUN6 antibody (AA 40-400)

Catalog No. ABIN7601646

This Rabbit Polyclonal antibody specifically detects NSUN6 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NSUN6 antibody (AA 40-400) (ABIN7601646)

Target: NSUN6 (NOP2/Sun Domain Family, Member 6 (NSUN6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-NSUN6 Antibody

Binding Specificity: AA 40-400

Purpose: Anti-NSUN6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NSUN6 Antibody Picoband® (ABIN7601646). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSUN6 recombinant protein (Position: E40-E400). Human NSUN6 shares 82.3% amino acid (aa) sequence identity with mouse NSUN6.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Chi, L., Delgado-Olguin, P. Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase family genes in early mouse embryogenesis. Gene Expr. Patterns 13: 319-327, 2013. 2. Gross, M. B. Personal Communication. Baltimore, Md. 11/10/2016. 3. Haag, S., Warda, A. S., Kretschmer, J., Gunnigmann, M. A., Hobartner, C., Bohnsack, M. T. NSUN6 is a human RNA methyltransferase that catalyzes formation of m(5)C72 in specific tRNAs. RNA 21: 1532-1543, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NSUN6

Target: NSUN6 (NOP2/Sun Domain Family, Member 6 (NSUN6))

Alternative Name: NSUN6

Background:

Synonyms: NSUN6, NOPD1, tRNA, cytosine(72-C(5-methyltransferase NSUN6, EC 2.1.1.-, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6

Background: Enables tRNA (cytidine-5-)-methyltransferase activity and tRNA binding activity. Involved in tRNA C5-cytosine methylation. Located in cytoplasm. Implicated in autosomal recessive intellectual developmental disorder 82.

Molecular Weight: 52 kDa

Gene ID: 221078