NSUN6 antibody (AA 40-400)

Catalog No. ABIN7601646

Product Details anti-NSUN6 Antibody

Target: NSUN6 (NOP2/Sun Domain Family, Member 6 (NSUN6))

Binding Specificity: AA 40-400

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Anti-NSUN6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NSUN6 Antibody Picoband® (ABIN7601646). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSUN6 recombinant protein (Position: E40-E400). Human NSUN6 shares 82.3% amino acid (aa) sequence identity with mouse NSUN6.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Chi, L., Delgado-Olguin, P. Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase family genes in early mouse embryogenesis. Gene Expr. Patterns 13: 319-327, 2013. 2. Gross, M. B. Personal Communication. Baltimore, Md. 11/10/2016. 3. Haag, S., Warda, A. S., Kretschmer, J., Gunnigmann, M. A., Hobartner, C., Bohnsack, M. T. NSUN6 is a human RNA methyltransferase that catalyzes formation of m(5)C72 in specific tRNAs. RNA 21: 1532-1543, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NSUN6

Target: NSUN6 (NOP2/Sun Domain Family, Member 6 (NSUN6))

Alternative Name: NSUN6 (NSUN6 Products)

Background:

Synonyms: NSUN6, NOPD1, tRNA, cytosine(72-C(5-methyltransferase NSUN6, EC 2.1.1.-, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6

Background: Enables tRNA (cytidine-5-)-methyltransferase activity and tRNA binding activity. Involved in tRNA C5-cytosine methylation. Located in cytoplasm. Implicated in autosomal recessive intellectual developmental disorder 82.

Molecular Weight: 52 kDa

Gene ID: 221078