Afamin antibody (AA 40-596)

Catalog No. ABIN7601653

The Rabbit Polyclonal anti-Afamin antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect Afamin in samples from Mouse and Rat.

Quick Overview for Afamin antibody (AA 40-596) (ABIN7601653)

Target: Afamin (AFM)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Afamin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-Afamin Antibody

Binding Specificity: AA 40-596

Purpose: Anti-Afm Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Afm Antibody Picoband® (ABIN7601653). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Afm recombinant protein (Position: D40-K596).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Belanger, L., Roy, S., Allard, D. New albumin gene 3-prime adjacent to the alpha-1-fetoprotein locus. J. Biol. Chem. 269: 5481-5484, 1994. 2. Lichenstein, H. S., Lyons, D. E., Wurfel, M. M., Johnson, D. A., McGinley, M. D., Leidli, J. C., Trollinger, D. B., Mayer, J. P., Wright, S. D., Zukowski, M. M. Afamin is a new member of the albumin, alpha-fetoprotein, and vitamin D-binding protein gene family. J. Biol. Chem. 269: 18149-18154, 1994. 3. Nishio, H., Dugaiczyk, A. Complete structure of the human alpha-albumin gene, a new member of the serum albumin multigene family. Proc. Nat. Acad. Sci. 93: 7557-7561, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Afamin

Target: Afamin (AFM)

Alternative Name: Afm

Background:

Synonyms: Agouti-related protein, Agrp, Agrt, Art

Tissue Specificity: Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.

Background: Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.

Molecular Weight: 87 kDa

Gene ID: 280662

UniProt: O89020