IRAK4 antibody (AA 419-460)

Catalog No. ABIN7601687

This anti-IRAK4 antibody is a Rabbit Polyclonal antibody detecting IRAK4 in WB and ELISA. Suitable for Human.

Quick Overview for IRAK4 antibody (AA 419-460) (ABIN7601687)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IRAK4 Antibody

Binding Specificity: AA 419-460

Purpose: Anti-IRAK4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IRAK4 Antibody Picoband® (ABIN7601687). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IRAK4 recombinant protein (Position: N419-S460).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Day, N., Tangsinmankong, N., Ochs, H., Rucker, R., Picard, C., Casanova, J.-L., Haraguchi, S., Good, R. Interleukin receptor-associated kinase (IRAK-4) deficiency associated with bacterial infections and failure to sustain antibody responses. J. Pediat. 144: 524-526, 2004. 2. Li, S., Strelow, A., Fontana, E. J., Wesche, H. IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase. Proc. Nat. Acad. Sci. 99: 5567-5572, 2002. 3. Scott, A. F. Personal Communication. Baltimore, Md. 4/25/2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4

Background:

Synonyms: Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57, p57Kip2, CDKN1C, KIP2

Tissue Specificity: Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver.

Background: IRAK-4(interleukin-1 receptor-associated kinase 4), also called REN64, in the IRAK family, is a protein kinase involved in signaling innate immune responses from Toll-like receptors. It also supports signaling from T-cell receptors. Scott(2002) mapped the REN64/IRAK4 gene to chromosome 12 based on similarity between the REN64 sequence(GenBank AF155118) and a chromosome 12 clone(GenBank AC093012). Functional analysis by Li et al.(2002) determined that IRAK4, like IRAK1 and Pelle, has auto- and cross-phosphorylation kinase activity. Precipitation and binding analyses showed weak interaction between IRAK4 and IRAK1, but IRAK4 did not interact with other IRAK family members. Overexpressed IRAK4 interacted with MYD88 and TRAF6 and activated mitogen-activated protein kinase(MAPK) and nuclear factor kappa-B(NFKB) pathways.

Molecular Weight: 52 kDa

Gene ID: 51135

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades