HSD17B8 antibody (AA 42-244)

Catalog No. ABIN7601689

The Rabbit Polyclonal anti-HSD17B8 antibody has been validated for WB, ELISA and FACS. It is suitable to detect HSD17B8 in samples from Human, Mouse and Rat.

Quick Overview for HSD17B8 antibody (AA 42-244) (ABIN7601689)

Target: HSD17B8 (Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-HSD17B8 Antibody

Binding Specificity: AA 42-244

Purpose: Anti-HSD17B8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HSD17B8 Antibody Picoband® (ABIN7601689). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HSD17B8 recombinant protein (Position: D42-D244).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ando, A., Kikuti, Y. Y., Shigenari, A., Kawata, H., Okamoto, N., Shiina, T., Chen, L., Ikemura, T., Abe, K., Kimura, M., Inoko, H. cDNA cloning of the human homologues of the mouse Ke4 and Ke6 genes at the centromeric end of the human MHC region. Genomics 35: 600-602, 1996. 2. Aziz, N., Anderson, E., Lee, G. Y., Woo, D. D. L. Arrested testis development in the cpk mouse may be the result of abnormal steroid metabolism. Molec. Cell. Endocr. 171: 83-88, 2001. 3. Chen, Z., Kastaniotis, A. J., Miinalainen, I. J., Rajaram, V., Wierenga, R. K., Hiltunen, J. K. 17-beta-hydroxysteroid dehydrogenase type 8 and carbonyl reductase type 4 assemble as a ketoacyl reductase of human mitochondrial FAS. FASEB J. 23: 3682-3691, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HSD17B8

Target: HSD17B8 (Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8))

Alternative Name: HSD17B8

Background:

Synonyms: Transcription factor Sp6, Krueppel-like factor 14, SP6, KLF14

Tissue Specificity: Ubiquitous.

Background: Estradiol 17 beta-dehydrogenase 8 is an enzyme that in humans is encoded by the HSD17B8 gene. In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.

Molecular Weight: 34 kDa

Gene ID: 7923

UniProt: Q92506

Pathways: Steroid Hormone Biosynthesis