Calpain 3 antibody (AA 42-764)

Catalog No. ABIN7601700

This Rabbit Polyclonal antibody specifically detects Calpain 3 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for Calpain 3 antibody (AA 42-764) (ABIN7601700)

Target: Calpain 3 (CAPN3)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Calpain 3 Antibody

Binding Specificity: AA 42-764

Purpose: Anti-Calpain 3/CAPN3 Antibody

Characteristics: Anti-Calpain 3/CAPN3 Antibody (ABIN7601700). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Calpain 3/CAPN3 recombinant protein (Position: I42-D764). Human CAPN3 shares 93.5% and 94.2% amino acid (aa) sequence identity with mouse and rat CAPN3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Calpain 3

Target: Calpain 3 (CAPN3)

Alternative Name: Calpain 3/CAPN3

Background: Calpain-3 is a protein that in humans is encoded by the CAPN3 gene. Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed.

Molecular Weight: 99 kDa

Gene ID: 825

UniProt: P20807

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development