Uromodulin antibody (AA 43-618)

Catalog No. ABIN7601726

This anti-Uromodulin antibody is a Rabbit Polyclonal antibody detecting Uromodulin in WB, IHC, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for Uromodulin antibody (AA 43-618) (ABIN7601726)

Target: Uromodulin (UMOD)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Uromodulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-Uromodulin Antibody

Binding Specificity: AA 43-618

Purpose: Anti-UMOD Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-UMOD Antibody Picoband® (ABIN7601726). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human UMOD recombinant protein (Position: E43-S618).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bachmann, S., Mutig, K., Bates, J., Welker, P., Geist, B., Gross, V., Luft, F. C., Alenina, N., Bader, M., Thiele, B. J., Prasadan, K., Raffi, H. S., Kumar, S. Renal effects of Tamm-Horsfall protein (uromodulin) deficiency in mice. Am. J. Physiol. Renal Physiol. 288: F559-F567, 2005. 2. Bernascone, I., Janas, S., Ikehata, M., Trudu, M., Corbelli, A., Schaeffer, C., Rastaldi, M. P., Devuyst, O., Rampoldi, L. A transgenic mouse model for uromodulin-associated kidney diseases shows specific tubulo-interstitial damage, urinary concentrating defect and renal failure. Hum. Molec. Genet. 19: 2998-3010, 2010. 3. Dahan, K., Devuyst, O., Smaers, M., Vertommen, D., Loute, G., Poux, J. M., Viron, B., Jacquot, C., Gagnadoux, M. F., Chauveau, D., Buchler, M., Cochat, P., Cosyns, J. P., Mougenot, B., Rider, M. H., Antignac, C., Verellen-Dumoulin, C., Pirson, Y. A cluster of mutations in the UMOD gene causes familial juvenile hyperuricemic nephropathy with abnormal expression of uromodulin. J. Am. Soc. Nephrol. 14: 2883-2893, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Uromodulin

Target: Uromodulin (UMOD)

Alternative Name: UMOD

Background:

Synonyms: Collagen alpha-1 (XVIII) chain, Endostatin, COL18A1

Tissue Specificity: Present in multiple organs with highest levels in liver, lung and kidney.

Background: Uromodulin (UMOD), also known as Tamm-Horsfall protein (THP), is a glycoprotein that in humans is encoded by the UMOD gene. The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants.

Molecular Weight: 90-110 kDa

Gene ID: 7369

UniProt: P07911