NCEH1 antibody (AA 44-352)

Catalog No. ABIN7601743

The Rabbit Polyclonal anti-NCEH1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect NCEH1 in samples from Human.

Quick Overview for NCEH1 antibody (AA 44-352) (ABIN7601743)

Target: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCEH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NCEH1 Antibody

Binding Specificity: AA 44-352

Purpose: Anti-AADACL1/NCEH1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AADACL1/NCEH1 Antibody Picoband® (ABIN7601743). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AADACL1/NCEH1 recombinant protein (Position: Q44-D352).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chiang, K. P., Niessen, S., Saghatelian, A., Cravatt, B. F. An enzyme that regulates ether lipid signaling pathways in cancer annotated by multidimensional profiling. Chem. Biol. 13: 1041-1050, 2006. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 1/27/2010. 3. Nagase, T., Kikuno, R., Ishikawa, K., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 65-73, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NCEH1

Target: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Alternative Name: NCEH1

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component, ARC36, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component, DRIP36, MED4, ARC36, DRIP36, VDRIP, HSPC126

Background: Neutral cholesterol ester hydrolase 1 (NCEH) also known as arylacetamide deacetylase-like 1 (AADACL1) or KIAA1363 is an enzyme that in humans is encoded by the NCEH1 gene. Predicted to enable hydrolase activity. Predicted to be involved in ether lipid metabolic process. Predicted to act upstream of or within SMAD protein signal transduction, protein dephosphorylation, and xenobiotic metabolic process. Located in membrane.

Molecular Weight: 46 kDa

Gene ID: 57552