FEZF1 antibody (AA 45-258)

Catalog No. ABIN7601763

This Rabbit Polyclonal antibody specifically detects FEZF1 in WB, ELISA, ICC, FACS and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FEZF1 antibody (AA 45-258) (ABIN7601763)

Target: FEZF1 (FEZ Family Zinc Finger 1 (FEZF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEZF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-FEZF1 Antibody

Binding Specificity: AA 45-258

Purpose: Anti-FEZF1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FEZF1 Antibody Picoband® (ABIN7601763). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FEZF1 recombinant protein (Position: A45-K258).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hirata, T., Nakazawa, M., Muraoka, O., Nakayama, R., Suda, Y., Hibi, M. Zinc-finger genes Fez and Fez-like function in the establishment of diencephalon subdivisions. Development 133: 3993-4004, 2006. 2. Kotan, L. D., Hutchins, B. I., Ozkan, Y., Demirel, F., Stoner, H., Cheng, P. J., Esen, I., Gurbuz, F., Bicakci, Y. K., Mengen, E., Yuksel, B., Wray, S., Topaloglu, A. K. Mutations in FEZF1 cause Kallmann syndrome. Am. J. Hum. Genet. 95: 326-331, 2014. 3. Song, I.-S., Oh, N. S., Kim, H.-T., Ha, G.-H., Jeong, S.-Y., Kim, J.-M., Kim, D.-I., Yoo, H.-S., Kim, C.-H., Kim, N.-S. Human ZNF312b promotes the progression of gastric cancer by transcriptional activation of the K-ras gene. Cancer Res. 69: 3131-3139, 2009. Note: Erratum: Cancer Res. 69: 4092 only, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FEZF1

Target: FEZF1 (FEZ Family Zinc Finger 1 (FEZF1))

Alternative Name: FEZF1

Background:

Synonyms: One cut domain family member 2, Hepatocyte nuclear factor 6-beta, HNF-6-beta, One cut homeobox 2, Transcription factor ONECUT-2, OC-2, ONECUT2, HNF6B

Tissue Specificity: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Background: FEZ family zinc finger 1 is a protein that in humans is encoded by the FEZF1 gene. This gene encodes a transcriptional repressor that belongs to the zinc finger double domain protein family. The encoded protein is thought to play a role in the embryonic migration of gonadotropin-releasing hormone neurons into the brain. Mutations in this gene are associated with hypogonadotropic hypogonadism-22 with anosmia. Alternative splicing results in multiple transcript variants.

Molecular Weight: 65 kDa

Gene ID: 389549