SPRED1 antibody (AA 45-322)

Catalog No. ABIN7601769

This Rabbit Polyclonal antibody specifically detects SPRED1 in WB, ELISA, FACS, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for SPRED1 antibody (AA 45-322) (ABIN7601769)

Target: SPRED1 (Sprouty-Related, EVH1 Domain Containing 1 (SPRED1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRED1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SPRED1 Antibody

Binding Specificity: AA 45-322

Purpose: Anti-SPRED1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SPRED1 Antibody (ABIN7601769). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPRED1 recombinant protein (Position: F45-K322). Human SPRED1 shares 89.9% amino acid (aa) sequence identity with mouse SPRED1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SPRED1

Target: SPRED1 (Sprouty-Related, EVH1 Domain Containing 1 (SPRED1))

Alternative Name: SPRED1

Background:

Synonyms: SPRED1, Sprouty-related, EVH1 domain-containing protein 1, Spred-1, hSpred1

Background: The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).

Molecular Weight: 50 kDa

Gene ID: 161742

UniProt: Q7Z699

Pathways: Positive Regulation of Response to DNA Damage Stimulus