PDIA5 antibody (AA 46-481)

Catalog No. ABIN7601801

This anti-PDIA5 antibody is a Rabbit Polyclonal antibody detecting PDIA5 in WB, ELISA, IHC, ICC, IF and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for PDIA5 antibody (AA 46-481) (ABIN7601801)

Target: PDIA5 (Protein Disulfide Isomerase Associated 5 (PDIA5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDIA5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PDIA5 Antibody

Binding Specificity: AA 46-481

Purpose: Anti-PDIA5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PDIA5 Antibody Picoband® (ABIN7601801). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDIA5 recombinant protein (Position: R46-Y481).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 5/9/2016. 2. Hayano, T., Kikuchi, M. Molecular cloning of the cDNA encoding a novel protein disulfide isomerase-related protein (PDIR). FEBS Lett. 372: 210-214, 1995. 3. Higa, A., Taouji, S., Lhomond, S., Jensen, D., Fernandez-Zapico, M. E., Simpson, J. C., Pasquet, J.-M., Schekman, R., Chevet, E. Endoplasmic reticulum stress-activated transcription factor ATF6-alpha requires the disulfide isomerase PDIA5 to modulate chemoresistance. Molec. Cell. Biol. 34: 1839-1849, 2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PDIA5

Target: PDIA5 (Protein Disulfide Isomerase Associated 5 (PDIA5))

Alternative Name: PDIA5

Background:

Synonyms: Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, Interleukin-1 family member 6, IL-1F6, IL36A, FIL1E, IL1E, IL1F6

Tissue Specificity: Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin.

Background: This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. The N-terminal TRX-like domain is the primary binding site for the major ER chaperone calreticulin and possibly other proteins and substrates as well. Alternative splicing results in multiple protein- and non-protein-coding transcript variants.

Molecular Weight: 60 kDa

Gene ID: 10954

UniProt: Q14554

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis