GALNT12 antibody (AA 49-572)

Catalog No. ABIN7601856

This Rabbit Polyclonal antibody specifically detects GALNT12 in ELISA, WB, FACS, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for GALNT12 antibody (AA 49-572) (ABIN7601856)

Target: GALNT12 (UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 12 (GalNAc-T12) (GALNT12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALNT12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-GALNT12 Antibody

Binding Specificity: AA 49-572

Purpose: Anti-GALNT12 Antibody Picoband®

Characteristics: Anti-GALNT12 Antibody Picoband® (ABIN7601856). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GALNT12 recombinant protein (Position: E49-Q572). Human GALNT12 shares 86.8% amino acid (aa) sequence identity with mouse GALNT12.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Guda, K., Moinova, H., He, J., Jamison, O., Ravi, L., Natale, L., Lutterbaugh, J., Lawrence, E., Lewis, S., Willson, J. K. V., Lowe, J. B., Wiesner, G. L., and 10 others. Inactivating germ-line and somatic mutations in polypeptide N-acetylgalactosaminyltransferase 12 in human colon cancers. Proc. Nat. Acad. Sci. 106: 12921-12925, 2009. 2. Guo, J.-M., Zhang, Y., Cheng, L., Iwasaki, H., Wang, H., Kubota, T., Tachibana, K., Narimatsu, H. Molecular cloning and characterization of a novel member of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family, pp-GalNAc-T12. FEBS Lett. 524: 211-218, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GALNT12

Target: GALNT12 (UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 12 (GalNAc-T12) (GALNT12))

Alternative Name: GALNT12

Background: This gene encodes a member of a family of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases, which catalyze the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to a serine or threonine residue on a polypeptide acceptor in the initial step of O-linked protein glycosylation. Mutations in this gene are associated with an increased susceptibility to colorectal cancer.

Molecular Weight: 67 kDa

Gene ID: 79695