Neural Wiskott-Aldrich syndrome protein (WASL) (AA 5-211) antibody

Catalog No. ABIN7601871

This Rabbit Polyclonal antibody specifically detects in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Neural Wiskott-Aldrich syndrome protein (WASL) (AA 5-211) antibody (ABIN7601871)

Target: Neural Wiskott-Aldrich syndrome protein (WASL)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details

Binding Specificity: AA 5-211

Purpose: Anti-N WASP/WASL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-N WASP/WASL Antibody Picoband® (ABIN7601871). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human N WASP/WASL recombinant protein (Position: Q5-H211).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cotta-de-Almeida, V., Westerberg, L., Maillard, M. H., Onaldi, D., Wachtel, H., Meelu, P., Chung, U., Xavier, R., Alt, F. W., Snapper, S. B. Wiskott-Aldrich syndrome protein (WASP) and N-WASP are critical for T cell development. Proc. Nat. Acad. Sci. 104: 15424-15429, 2007. 2. Fukuoka, M., Miki, H., Takenawa, T. Identification of N-WASP homologs in human and rat brain. Gene 196: 43-48, 1997. 3. Guenin-Mace, L., Veyron-Churlet, R., Thoulouze, M.-I., Romet-Lemonne, G., Hong, H., Leadlay, P. F., Danckaert, A., Ruf, M.-T., Mostowy, S., Zurzolo, C., Bousso, P., Chretien, F., Carlier, M.-F., Demangel, C. Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation. J. Clin. Invest. 123: 1501-1512, 2013.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details

Target: Neural Wiskott-Aldrich syndrome protein (WASL)

Alternative Name: WASL

Background:

Synonyms: POU domain, class 4, transcription factor 1

Tissue Specificity: Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.

Background: Neural Wiskott-Aldrich syndrome protein is a protein that in humans is encoded by the WASL gene. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension.

Molecular Weight: 70 kDa

Gene ID: 8976

UniProt: O00401