NANP antibody (AA 5-246)

Catalog No. ABIN7601874

This Rabbit Polyclonal antibody specifically detects NANP in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NANP antibody (AA 5-246) (ABIN7601874)

Target: NANP (N-Acetylneuraminic Acid Phosphatase (NANP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NANP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-NANP Antibody

Binding Specificity: AA 5-246

Purpose: Anti-NANP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NANP Antibody Picoband® (ABIN7601874). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NANP recombinant protein (Position: R5-M246).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Maliekal, P., Vertommen, D., Delpierre, G., Van Schaftingen, E. Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase. Glycobiology 16: 165-172, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NANP

Target: NANP (N-Acetylneuraminic Acid Phosphatase (NANP))

Alternative Name: NANP

Background:

Synonyms: One cut domain family member 2, Hepatocyte nuclear factor 6-beta, HNF-6-beta, One cut homeobox 2, Transcription factor ONECUT-2, OC-2, ONECUT2, HNF6B

Tissue Specificity: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Background: NANP (N-acylneuraminate-9-phosphatase), also known as HDHD4 (Haloacid dehalogenase-like hydrolase domain-containing protein 4), is a 248 amino acid protein that belongs to the haloacid dehalogenase (HAD) family and is responsible for dephosphorylating Neu5Ac-9-phosphate to form N-acetylneuraminate. Characteristic of the HAD phosphatase family, the catalytic activity of NANP is dependent upon the presence of magnesium and is inhibited by vanadate and calcium.

Molecular Weight: 30 kDa

Gene ID: 140838