Pirin antibody (AA 5-266)

Catalog No. ABIN7601876

This anti-Pirin antibody is a Rabbit Polyclonal antibody detecting Pirin in ELISA, WB, IF, ICC and FACS. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for Pirin antibody (AA 5-266) (ABIN7601876)

Target: Pirin (PIR)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pirin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Pirin Antibody

Binding Specificity: AA 5-266

Purpose: Anti-PIR Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-PIR Antibody Picoband® (ABIN7601876). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PIR recombinant protein (Position: K5-Q266). Human PIR shares 96.6% and 95.8% amino acid (aa) sequence identity with mouse and rat PIR, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Monkey, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 8/27/2014. 2. Wendler, W. M. F., Kremmer, E., Forster, R., Winnacker, E.-L. Identification of pirin, a novel highly conserved nuclear protein. J. Biol. Chem. 272: 8482-8489, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Pirin

Target: Pirin (PIR)

Alternative Name: PIR

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: Pirin is a protein that in humans is encoded by the PIR gene. This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.

Molecular Weight: 36 kDa

Gene ID: 8544

UniProt: O00625