XPA antibody (AA 5-273)

Catalog No. ABIN7601877

This Rabbit Polyclonal antibody specifically detects XPA in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for XPA antibody (AA 5-273) (ABIN7601877)

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-XPA Antibody

Binding Specificity: AA 5-273

Purpose: Anti-XPA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-XPA Antibody Picoband® (ABIN7601877). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human XPA recombinant protein (Position: D5-M273).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Li L, Elledge SJ, Peterson CA, Bales ES, Legerski RJ (May 1994). "Specific association between the human DNA repair proteins XPA and ERCC1". Proceedings of the National Academy of Sciences of the United States of America. 91 (11): 5012-6. 2. Nagai A, Saijo M, Kuraoka I, Matsuda T, Kodo N, Nakatsu Y, Mimaki T, Mino M, Biggerstaff M, Wood RD (Jun 1995). "Enhancement of damage-specific DNA binding of XPA by interaction with the ERCC1 DNA repair protein". Biochemical and Biophysical Research Communications. 211 (3): 960-6.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for XPA

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Alternative Name: XPA

Background:

Synonyms: Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110, HGS, HRS

Tissue Specificity: Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.

Background: DNA repair protein complementing XP-A cells is a protein that in humans is encoded by the XPA gene. This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 39 kDa

Gene ID: 7507

UniProt: P23025

Pathways: DNA Damage Repair