ALDOA antibody (AA 50-364)

Catalog No. ABIN7601908

The Mouse Monoclonal anti-ALDOA antibody has been validated for WB, IHC, IF, ICC and FACS. It is suitable to detect ALDOA in samples from Human.

Quick Overview for ALDOA antibody (AA 50-364) (ABIN7601908)

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALDOA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 6H8

Product Details anti-ALDOA Antibody

Binding Specificity: AA 50-364

Purpose: Anti-Aldolase/ALDOA Antibody Picoband® (monoclonal, 6H8)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Aldolase/ALDOA Antibody Picoband® (monoclonal, 6H8) (ABIN7601908). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Aldolase/ALDOA recombinant protein (Position: E50-Y364).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-μg/1x10⁶ cells, Human
1. Du, S, Guan, Z, Hao, L, Song, Y, Wang, L, Gong, L, Liu, L, Qi, X, Hou, Z, Shao, S (2014). "Fructose-bisphosphate aldolase a is a potential metastasis-associated marker of lung squamous cell carcinoma and promotes lung cell tumorigenesis and migration". PLOS ONE. 9 (1): e85804. 2. Yao, DC, Tolan, DR, Murray, MF, Harris, DJ, Darras, BT, Geva, A, Neufeld, EJ (15 March 2004). "Hemolytic anemia and severe rhabdomyolysis caused by compound heterozygous mutations of the gene for erythrocyte/muscle isozyme of aldolase, ALDOA(Arg303X/Cys338Tyr)". Blood. 103 (6): 2401-3. 3. St-Jean, M, Izard, T, Sygusch, J (11 May 2007). "A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein". The Journal of Biological Chemistry. 282 (19): 14309-15.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Alternative Name: ALDOA

Background:

Synonyms: Cofilin-2, Cofilin, muscle isoform, CFL2

Tissue Specificity: Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.

Background: Aldolase A (ALDOA, or ALDA), also known as fructose-bisphosphate aldolase, is an enzyme that in humans is encoded by the ALDOA gene on chromosome 16. This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10.

Molecular Weight: 39 kDa

Gene ID: 226

UniProt: P04075

Pathways: Ribonucleoside Biosynthetic Process