TFEB antibody (AA 50-461)

Catalog No. ABIN7601914

The Rabbit Polyclonal anti-TFEB antibody has been validated for WB and ELISA. It is suitable to detect TFEB in samples from Human, Mouse and Rat.

Quick Overview for TFEB antibody (AA 50-461) (ABIN7601914)

Target: TFEB (Transcription Factor EB (TFEB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFEB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TFEB Antibody

Binding Specificity: AA 50-461

Purpose: Anti-TFEB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TFEB Antibody Picoband® (ABIN7601914). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TFEB recombinant protein (Position: T50-A461).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Argani, P., Hawkins, A., Griffin, C. A., Goldstein, J. D., Haas, M., Beckwith, J. B., Mankinen, C. B., Perlman, E. J. A distinctive pediatric renal neoplasm characterized by epithelioid morphology, basement membrane production, focal HMB45 immunoreactivity, and t(6,11)(p21.1,q12) chromosome translocation. Am. J. Path. 158: 2089-2096, 2001. 2. Carr, C. S., Sharp, P. A. A helix-loop-helix protein related to the immunoglobulin E box-binding proteins. Molec. Cell. Biol. 10: 4384-4388, 1990. 3. Davis, I. J., Hsi, B.-L., Arroyo, J. D., Vargas, S. O., Yeh, Y. A., Motyckova, G., Valencia, P., Perez-Atayde, A. R., Argani, P., Ladanyi, M., Fletcher, J. A., Fisher, D. E. Cloning of an alpha-TFEB fusion in renal tumors harboring the t(6,11)(p21,q13) chromosome translocation. Proc. Nat. Acad. Sci. 100: 6051-6056, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TFEB

Target: TFEB (Transcription Factor EB (TFEB))

Alternative Name: TFEB

Background:

Synonyms: NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor, CD314, KLRK1, D12S2489E, NKG2D

Tissue Specificity: Expressed in natural killer (NK) cells, CD8 (+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs).

Background: Transcription factor EB is a protein that in humans is encoded by the TFEB gene. TFEB is a master gene for lysosomal biogenesis. It encodes a transcription factor that coordinates expression of lysosomal hydrolases, membrane proteins and genes involved in autophagy. Upon nutrient depletion and under aberrant lysosomal storage conditions such as in lysosomal storage diseases, TFEB translocates from the cytoplasm to the nucleus, resulting in the activation of its target genes. TFEB overexpression in cultured cells induces lysosomal biogenesis, exocytosis and autophagy. Viral-mediated TFEB overexpression in cellular and mouse models of lysosomal storage disorders and in common neurodegenerative diseases such as Huntington, Parkinson and Alzheimer diseases, resulted in intracellular clearance of accumulating molecules and rescue of disease phenotypes. TFEB is activated by PGC1-alpha and promotes reduction of htt aggregation and neurotoxicity in a mouse model of Huntington disease. TFEB overexpression has been found in patients with renal cell carcinoma and pancreatic cancer and was shown to promote tumorogenesis via induction of varius oncogenic signals.

Molecular Weight: 70 kDa

Gene ID: 7942

UniProt: P19484