ECD/SGT1 antibody (AA 50-632)

Catalog No. ABIN7601917

The Rabbit Polyclonal anti-ECD/SGT1 antibody is suitable to detect ECD/SGT1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for ECD/SGT1 antibody (AA 50-632) (ABIN7601917)

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECD/SGT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ECD/SGT1 Antibody

Binding Specificity: AA 50-632

Purpose: Anti-SGT1/ECD Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SGT1/ECD Antibody (ABIN7601917). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ECD recombinant protein (Position: L50-N632).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ECD/SGT1

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Alternative Name: ECD

Background:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 10, ADAM 10, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease, CD156c, ADAM10, KUZ, MADM

Tissue Specificity: Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.

Background: Protein SGT1 is a protein that in humans is encoded by the ECD gene. Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.

Molecular Weight: 73 kDa

Gene ID: 11319

UniProt: O95905

Pathways: Regulation of Carbohydrate Metabolic Process