Neuropilin 1 antibody (AA 504-827)

Catalog No. ABIN7601921

This anti-Neuropilin 1 antibody is a Mouse Monoclonal antibody detecting Neuropilin 1 in WB, ICC, FACS and IF. Suitable for Human and Rat.

Quick Overview for Neuropilin 1 antibody (AA 504-827) (ABIN7601921)

Target: Neuropilin 1 (NRP1)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neuropilin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 8G6G7

Product Details anti-Neuropilin 1 Antibody

Binding Specificity: AA 504-827

Purpose: Anti-Neuropilin 1 Antibody Picoband® (monoclonal, 8G6G7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Neuropilin 1 Antibody Picoband® (monoclonal, 8G6G7) (ABIN7601921). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively.

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Gagnon, M. L., Bielenberg, D. R., Gechtman, Z., Miao, H.-Q., Takashima, S., Soker, S., Klagsbrun, M. Identification of a natural soluble neuropilin-1 that binds vascular endothelial growth factor: in vivo expression and antitumor activity. Proc. Nat. Acad. Sci. 97: 2573-2578, 2000. 2. Gu, C., Rodriguez, E. R., Riemert, D. V., Shu, T., Fritzsch, B., Richards, L. J., Kolodkin, A. L., Ginty, D. D. Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev. Cell 5: 45-57, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Neuropilin 1

Target: Neuropilin 1 (NRP1)

Alternative Name: NRP1

Background:

Synonyms: Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, RF-A protein 1, Single-stranded DNA-binding protein, Replication protein A 70 kDa DNA-binding subunit, N-terminally processed, RPA1, REPA1, RPA70

Tissue Specificity: Expressed in lung fibroblasts (at protein level). Expressed in monocytes. Highly expressed in liver, also found in kidney and brain.

Background: This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contain a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors, they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Molecular Weight: 120 kDa

Gene ID: 8829

UniProt: O14786

Pathways: Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals