PIWIL4 antibody (AA 51-749)

Catalog No. ABIN7601940

This Rabbit Polyclonal antibody specifically detects PIWIL4 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for PIWIL4 antibody (AA 51-749) (ABIN7601940)

Target: PIWIL4 (Piwi-Like 4 (PIWIL4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIWIL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PIWIL4 Antibody

Binding Specificity: AA 51-749

Purpose: Anti-PIWIL4/PIWI Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PIWIL4/PIWI Antibody (ABIN7601940). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PIWIL4/PIWI recombinant protein (Position: R51-N749).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PIWIL4

Target: PIWIL4 (Piwi-Like 4 (PIWIL4))

Alternative Name: PIWIL4

Background:

Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2

Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.

Background: PIWI-like protein 4 (PIWIL4) is a 97 kDa member of the argonaute family of proteins and the PIWI subfamily. Human PIWIL4 is 852 amino acids (aa) in length and contains one PAZ domain (aa 268-384) and one PIWI domain (aa 546-838). There are three isoforms for human PIWIL4. Isoform 1 is the standard protein. Isoform 2 has a two aa substitution for aa 1-29 in isoform 1 and a deletion of aa 523-852. Isoform 3 has a deletion of aa 1-69 and a deletion of aa 523-852. Human PIWIL4 shares 77 % aa sequence identity with mouse and rat PIWIL4. PIWIL4 plays a central role in spermatogenesis.

Molecular Weight: 97 kDa

Gene ID: 143689

UniProt: Q7Z3Z4